| Single-cell transcriptomics reveals a compartmentalized antiviral interferon response in the nasal epithelium of mice |
| 論文作者 |
Wang, XF; Dong, M; Wu, XC; Schnepf, D; Thiel, J; Sun, WF; Wolfrum, C; Li, SS; Jin, WF; Staeheli, P; Ye, L |
| 期刊/會(huì)議名稱 |
JOURNAL OF VIROLOGY |
| 論文年度 |
2025 |
| 論文類別 |
|
| 摘要 |
Type III interferons (IFNs) primarily act on epithelial cells and protect against virus infection of the mucosa, whereas type I IFNs act more systemically. To date, it has been unknown which epithelial subtypes in the upper airways, the primary site for initial infection for most respiratory viruses, primarily rely on type III IFN or type I IFNs for antiviral protection. To address this question, we performed a single-cell transcriptomics analysis of the epithelial IFN-mediated response focusing on the upper airways of mice. This work identified nine distinct cell types derived from the olfactory epithelium and thirteen distinct cell types from the respiratory epithelium. Interestingly, type I IFNs induced a stronger antiviral transcriptional response than type III IFN in respiratory epithelial cells, whereas in olfactory epithelial cells, including sustentacular (SUS) and Bowman's gland cells (BGC), type III IFN was more dominant compared to type I IFN. SUS and BGC, which provide structural support and maintain the integrity of olfactory sensory neurons, were highly susceptible to infection with a mouse-adapted variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 MA20) but were protected against infection if the animals were prophylactically treated with type III IFN. These findings demonstrate a high degree of cell type heterogeneity in terms of interferon- mediated antiviral responses and reveal a potent role for type III IFNs in protecting the olfactory epithelium. |
| 卷 |
99 |
| 影響因子 |
3.8 |
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