caopornx在线超碰免费-欧美亚洲性色影视在线-人妻无码AV一区二区三区-欧美日韩国产一区二区三区播放-青青草原精品国产亚洲AV-日本黄A级A片国产免费-亚洲精品一区久久久久久-成人18禁在线WWW免费视频

Background: Epithelial-to-mesenchymal transition (EMT) in nasal epithelial cells is involved in tissue remodeling of chronic rhinosinusitis with nasal polyps (CRSwNP). Our study investigated the molecular mechanisms that microRNA-182 (miR-182) regulated EMT in eosinophilic (Eos) and non-eosinophilic (non-Eos) CRSwNP. Objective: To investigate the mechanism by which miR-182 regulates EMT in human nasal epithelial cells (hNEPCs). Methods: The expression of EMT markers (E-cadherin, N-cadherin and vimentin), transforming growth factor (TGF)-I31, and miR-182 were determined by western blotting and reverse transcription -quantitative PCR (RT-qPCR). Fluorescence in situ hybridization (FISH) was used to detect the miR-182 localization. Additionally, EMT markers expression and cell morphology changes were checked upon treatment with TGF-I31, or TGF-I31 with miR-182 inhibitor, or miR-182 mimics, or miR-182 inhibitor alone in hNEPCs. Results: In both Eos CRSwNP and non-Eos CRSwNP, the expression levels of E-cadherin were downregulated while the expression levels of N-cadherin, vimentin, TGF-I31 and miR-182 were significantly upregulated compared with control nasal tissues. Additionally, more significant changes in these EMT markers were observed in the Eos-CRSwNP when compared with the non-Eos CRSwNP. Invitro hNEPCs model, TGF-I31 upregulated miR-182 expression and promoted EMT in hNEPCs, indicated by changes in cell morphology and EMT markers expression. Furthermore, these upregulations were reversed by miR-182 inhibitor. Conclusion: This study showed that miR-182-induced EMT in response to TGF-I31 might promote nasal polypogenesis in both Eos CRSwNP and non-Eos CRSwNP, thus providing potential targets for the future development of novel therapeutic approaches for the management of CRSwNP.