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Isolation and Characterization of Extracellular Vesicles Secreted from Human Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cells.
論文作者 Wu, Qiang; Ke, Min-Xia; Yang, Huang-Tian
期刊/會(huì)議名稱 Methods in molecular biology (Clifton, N.J.)
論文年度 2022
論文類別
摘要 ? 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.Extracellular vesicles (EVs) secreted by human pluripotent stem cells-derived cardiovascular progenitor cells (hPSC-CVPCs) can improve repair of infarcted hearts in mouse and nonhuman primate myocardial infarction models. To fully achieve their values, it is essential to establish an efficient method for the isolation of EVs from hPSC-CVPCs. Here we describe the protocols for efficient isolation and characterization of EVs from the conditioned medium of hPSC-CVPCs.
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